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Natural Products Analysis

Bioactive Natural Product Analysis

Extraction & purification using MPLC (Multiple prep-LC).
Target & non-target analysis using UHPLC-Orbitrap high-resolution MS.
Rapid antioxidant analysis (ABTS, DPPH, TPC, TFC).
Installed equipments:

Vanquish Horizon UHPLC system

Thermo Fisher Scientific OrbitrapTM ID-X Tribrid MS

Thermo Fisher Scientific OrbitrapTM Exploris 120

SpectraMax iD3 Multi-Mode Microplate Reader

Natural Product Information Library

In-house Natural Product Library Development

Reliable standard compound with structural diversity.
Custom MS/MS spectrum database.
The library contains ESI MS/MS spectra of plant specialized metabolites.
Metabolites in the library are as structurally diverse as phytochemicals in ZINC15.
For comprehensiveness, data were recorded under various experimental conditions.
Data blocks defined by environment conditions were applied to the library search.
The BMDMS-NP with command-line executable source codes and record data are publicly available.

MS-based Compound Analysis

Analysis & Consulting for MSn-based
Compound Identification

LC-MS/MS (Liquid chromatography-tandem mass spectrometry) is a highly versatile analytical technique that can be used in a wide range of fields including:

Proteomics: LC-MS/MS can be used for the identification and quantification of proteins in complex biological samples, such as blood or tissue samples. It can be used to study the changes in protein expression in response to different conditions or treatments.

Metabolomics: LC-MS/MS can be used to analyze small molecules, such as metabolites, in biological samples. It can be used to identify and quantify metabolites in different biological systems and to study their role in biological processes.

Clinical research: LC-MS/MS can be used in clinical research to monitor drug levels in patient samples, to study drug metabolism and pharmacokinetics, and to identify potential biomarkers for diseases.

Environmental analysis: LC-MS/MS can be used to detect and quantify pollutants, such as pesticides and herbicides, in environmental samples. It can also be used to study the fate and transport of pollutants in different environmental matrices.

Food and beverage analysis: LC-MS/MS can be used to analyze food and beverage samples for the presence of contaminants, such as mycotoxins, and for the quantification of nutrients and other bioactive compounds.

Forensic analysis: LC-MS/MS can be used to detect and quantify drugs and other illicit substances in biological or environmental samples, such as blood or urine, and to provide evidence in criminal investigations.
Overall, LC-MS/MS is a powerful and versatile analytical tool that can be applied in many different fields to study a wide range of biological and environmental processes.

Biological Activity Analysis

SCREENING & ASSAY

In vitro antioxidant screening (ABTS, DPPH, TPC, TFC).
Cell-based assay (antioxidant, anti-obesity, anti-inflammation, neuroprotection, anti-sarcopenia etc.).
Neuroprotection:

Cell survival (MTT)

Intracellular antioxidant (DCFH-DA)

Fluorescence microscope (DCFH-DA)
Anti-inflammation:

Cell survival (MTT)

Nitrogen oxide assay

mRNA expression (TNF-α, IL cytokines..)
Anti-obesity:

Cell survival (MTT)

Triglyceride staining test (Oil Red O)
Anti-sarcopenia:

Cell survival (MTT)

Muscle staining (H&E)

Muscle growth factor (Mst1, atrogin..)
Installed equipments:

Teledyne ISCO CombFlash NextGen 300+

SpectraMax iD3 Multi-Mode Microplate Reader